Bioinformatics scientist Interview Questions
170
Bioinformatics Scientist interview questions shared by candidates
Can you manipulate bclfastq files?
2 Answers↳
I described the pipeline I constructed.
↳
lots of script can do bclfastq, why ask this question?
Do you feel you would fit in a culture of rapid-change?
2 Answers
Asking me about NGS experience, general and w/ PacBio.
1 Answers↳
Described NGS functions in previous jobs.
Have you ever encountered batch effect or any technical effect. What was the impact on your data and how do you control for these unwanted variables.
1 Answers↳
Use the ComBat function from the SVA Bioconductor package to combat batch effect
Some coding and statistics questions, some questions about NGS pipelines.
1 Answers↳
Did you have to do a drug test?
What distribution is single-cell RNA-seq data? Why is it that distribution? How do you deal with integrating multiple data sets with different distribution.
1 Answers↳
Negative binomial distribution
NGS data analysis workflow/pipeline and tools
1 Answers↳
The current "best" tools for differential expression analysis, e.g. DESeq2 and EdgeR, incorporate normalization factors accounting for library size and composition. Pointedly, these tools do not "just" normalize prior to analysis, they include the normalization factors as features within the model. If you are looking to utilize a neural network, hierarchical bayesian, or other type of machine learning approach to differential expression, determining the appropriate method of normalization will be a make or break point. Less
